TABLE 2.
Characterization of transposome insertion derivatives of A. baumannii 19606
| Insertion | Target site | Gene, function disrupteda |
|---|---|---|
| 1 | GCCCTAAAA | dapA, dihydrodipicolinate synthase |
| 2 | GATCATAGT | gacS/lemA, histidine kinase sensor |
| 4 | GTAGTGACT | csuB, chaperone-usher secretion system |
| 5 | CTACAATCA | gacS/lemA, histidine kinase sensor |
| 9 | CGCGGATAC | trpD, anthranilate phosphoribosyltransferase |
| 10 | GTTTATTCA | argF, ornithine carbamoyltransferase |
| 11 | GAACATGAT | gacS/lemA, histidine kinase sensor |
| 12 | GGGCCATAC | argG, argininosuccinate synthase |
| 13 | ATAGAATGG | aceElaceA, pyruvate dehydrogenase E1 |
| 15 | AATGGAAAC | proA, γ-glutamyl phosphate reductase |
| 16 | AATATACGT | cysI/nirA/sir, sulfite reductase |
| 19 | CTACGATGC | trpE, anthranilate synthase |
| 23 | CTATTCACA | trpE, anthranilate synthase |
| 25 | GAACATGAT | gacS/lemA, histidine kinase sensor |
| 26 | CATGTGAAT | cysI/nirA/sir, sulfite reductase |
| 28 | CATGTGAAT | cysI/nirA/sir, sulfite reductase |
| 30 | GCATTGGGT | hisA/his4, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase |
| 31 | CTGCAAACC | hisH, glutamine amidotransferase |
| 44 | GTTTTACGT | cysI/nirA/sir, sulfite reductase |
| 144 | GTCACAAAC | csuE, chaperone-usher secretion system |
| 249 | ATGGTAAAA | Hypothetical sensor histidine kinase |
a
Potential genes and functions were predicted by BLASTp and BLASTx searches.