TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| CC118 λ pir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λ pir lysogen | 20 |
| HB101 | recA thi pro leu hsd M+ Smr | 24 |
| MT102 | araD139 (ara-leu)7697 Δlac thi hsdR | Laboratory collection |
| XL1Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)] | Stratagene |
| P. putida | ||
| IsoF | Wild-type isolate from tomato roots | 45 |
| F117 | KmrppuI::npt mutant of IsoF | This study |
| IsoF-D4 | ppuA::luxAB mutant of IsoF | This study |
| F117-D1 | KmrppuI::npt ppuA::luxAB double mutant of IsoF | This study |
| Plasmids | ||
| pAS-L22 | pGA-L14 containing a PppuI-luxAB transcriptional fusion | This study |
| pBBR1MCS-2 | Broad-host-range plasmid, lacZα; Kanr | 27 |
| pBBR1MCS-5 | Broad-host-range plasmid, lacZα; Gmr | 27 |
| pBK-miniTn7-gfp3 | pUC19-based delivery plasmid for miniTn7-gfp3; Kmr Apr Cmr Smrmob+ | 25 |
| pEX18Gm | GmroriT+sacB+, gene replacement vector with multiple-cloning site from pUC18 | 21 |
| pEXF1 | Gmr Kmr, pEX18Gm derivative for inactivation of ppuI | This study |
| pEXF2 | Gmr, pEX18Gm derivative for inactivation of ppuA | This study |
| pGA-L14 | Broad-host-range luxAB-based promoter probe vector | Laboratory collection |
| pGEM-3Zf(+) | lacZα, cloning vector; Apr | Promega, Madison, Wis. |
| pGF1 | 2.9-kb SalI chromosomal fragment containing ppuI-rsaL-ppuR of IsoF cloned into pGEM-3Zf(+) | This study |
| pGF3 | 1.4-kb EcoRI chromosomal fragment containing ppuI of IsoF cloned into pBBR1MCS-5 | This study |
| pGF4 | Kmr, 13-kb SacI chromosomal fragment containing the ppu locus of F117 plus flanking regions cloned into pGEM-3Zf(+) | This study |
| pKR-C12 | AHL sensor plasmid; pBBR1MCS-5 carrying PlasB-gfp(ASV)-Plac-lasR; Gmr | 39 |
| pPPUR1 | pGEM-3Zf(+) containing the ppuR gene of IsoF | This study |
| pRK600 | ColE1 RK2-Mob+ RK2-Tra+; helper plasmid; Cmr | 12 |
| pSB403 | Bioluminescent AHL sensor plasmid; Tetr | 53 |
| pUX-BF13 | R6K replicon-based helper plasmid, providing the Tn7 transposition function in trans, Aprmob+ | 2 |