TABLE 1.

Similarities of sequences obtained from excised DGGE bands (see Fig. 4) to sequences in the EMBL database, Gram status and phylogenetic position of the OTU, and response of the band intensity to presence of protozoa in microcosms amended with wheat root material or nutrient solution

Band	Sequencea	Accession no.	Nearest relativeb	Accession no.c	Similarity (%)	Phylogenetic group	Gram status	Response to grazingd
								Roots	Nut. sol.
A	1	AJ488008	Uncultured bacterium clone P3 (1)	AF414577	98.7	CFB groupe	G−	−	−
	2	AJ488009	Uncultured bacterium clone P3 (1)	AF414577	98.1
B1	1	AJ488010	Flavobacterium (1)	AF375835	98.7	CFB group
	2	AJ488011	Uncultured Bacteriodaceae bacterium (1)	AJ318151	98.7		G−	ND	ND
B2		AJ488012	Flavobacterium (1); unidentified (1)	AY039834	100	CFB group
C1		AJ488013	Pseudomonas (7)	AF105387	98.7	γ Proteobacteria	G−	(+)	ND
C2		AJ488014	Pseudomonas (7)	AF105387	100	γ Proteobacteria
D		AJ488015	Bdellovibrio (1)	AF084853	98.8	δ Proteobacteria	G−	−	−
E		AJ488016	Buttiauxella (3); Enterobacter (4); Hafnia (1); Obesumbacterium (1)	AJ233406	100	γ Proteobacteria	G−	−	−
F1		AJ488017	Bdellovibrio bacteriovorous (15); Bdellovibrio (6)	AF084850	100	δ Proteobacteria	G−	−	−
F2		AJ488018	B. bacteriovorous (15); Bdellovibrio (6)	AF084850	100	δ Proteobacteria
G		AJ488019	Leifsonia poae (1); Agrococcus jenensis (1); unidentified (1); uncultured (1)	AF116342	96.7	High G + C gram positive	G+	ND	+
H		AJ488020	Achromobacter xylosoxidans (1); unidentified (1); uncultured (1)	AF225979	98.0	β Proteobacteria	G−	+	ND
I		AJ488021	Arthrobacter (27); Methylomicrobium (1); uncultured (4); unidentified (2)	X83408	100	High G + C gram positive	G+	ND	+
J1		AJ488022	Arthrobacter rhombi (1)	Y15884	99.3	High G + C gram positive	G+	+	no
J2		AJ488023	Arthrobacter (13); Brachybacterium (8); Brevibacterium (1); unidentified (1)	M23411	100	High G + C gram positive
K1		AJ488024	Arthrobacter (13); Brachybacterium (8); Brevibacterium (1); unidentified (1)	M23411	100	High G + C gram positive	G+	ND	+
K2	1	AJ488025	Arthrobacter (13); Brachybacterium (8); Brevibacterium (1); unidentified (1)	M23411	100	High G + C gram positive
	2	AJ488026	Arthrobacter psychrolactophilus (1)	AF134181	96.7
L1	1	AJ488027	Arthrobacter (13); Brachybacterium (2); Brevibacterium (1); unidentified (1)	M23411	98.6	High G + C gram positive	G+	ND	+
	2	AJ488028	A. psychrolactophilus (1)	AF134181	95.9
L2	1	AJ488029	Arthrobacter (13); Brachybacterium (8); Brevibacterium (1); unidentified (1)	M23411	100	High G + C gram positive
	2	AJ488030	A. psychrolactophilus (1)	AF134181	96.6
M1		AJ488031	Alcaligenes (1)	X92415	97.5	β Proteobacteria	G−	ND	−
M2		AJ488032	Alcaligenes (1)	X92415	96.2	β Proteobacteria

^aFrom some bands, two separate bands were obtained after rerunning the excised band. In these cases, sequences from both bands are included.

^bNumber of hits to a species or genus with the indicated similarity is given in parentheses.

^cAccession number of the nearest relative. When more than one sequence had the same similarity, only the accession number of the first sequence is given.

^dEffect of the presence of protozoa on the intensity of the band in microcosms amended with the two types of substrate. The effect was evaluated by two-way analysis of variance on the percent intensity of the bands. +, significant positive effect of grazing (P < 0.01); −, significant negative effect of grazing; (+), discernible but nonsignificant positive effect; no, no effect of grazing; ND, not detectable due to very low or variable band intensity. Nut. sol., nutrient solution.

^eCFB, Cytophaga-Flavobacterium.