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An example of restriction enzyme digestion on randomly chosen colonies from the MURA library. E. coli colonies were randomly selected from the library, and their plasmids were isolated. Each sample of plasmid DNA was digested with EcoRI and SalI and then examined by electrophoresis in a 2.5% Metaphore agarose gel (FMC). Lane M, λ/EcoRI and HindIII digests and a 100-bp ladder DNA size marker; lanes 1 to 13, EcoRI and SalI digests of each plasmid.
