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Size distribution of the MURA libraries. The truncated gene sizes of 100 randomly chosen variants in MURA libraries were determined by restriction digestion of each sample. The digest samples were estimated by agarose gel electrophoresis (2.5% Metaphore agarose gel) and the gel analysis software package Quantity One (Bio-Rad). The calculated sizes of the insert genes of randomly selected members were arranged in the order of ascending size.
