TABLE 2.

Effect of P. luminescens HGB008 phenotypic variation on ADF activity at California test sites

Test conditiona	Strainb	Form	ADF activityc
Field in vitro	E. coli	NAg	0.95 ± 0.11 Ad
	HGB008	1°	0.08 ± 0.01 Bd
	HGB159	2°	0.08 ± 0.02 Bd
Laboratory in vitro	E. coli	NA	1 Ae
	HGB008	1°	0.05 ± 0.01 Be
	HGB159	2°	0.05 ± 0.01 Be
Field in vivo	Control	NA	0.51 ± 0.08 Af
	HGB008	1°	0.019 ± 0.002 Bf
	HGB159	2°	0.47 ± 0.05 Af

^aSee Materials and Methods for detailed explanation of test conditions.

^bSee Table 1 for details on the strains. E. coli, E. coli (Migula). Control, freeze-killed insects used for in vivo experiments.

^cFor in vitro assays, values are the proportion of volume (in microliters) removed from wells. For in vivo studies, values are the mean proportion of ants visiting. Different letters after values indicate significant difference within an experiment (P < 0.05 by Duncan's new multiple-range test).

^dThere was a significant difference between the number of field ants visiting the E. coli control versus the bacterial treatments (F = 34.93, df = 2, 32; P < 0.05).

^eSignificantly more suspension remained in the wells of 1°- or 2°-form bacterial treatments than that for the E. coli control treatment (F = 4697.98, df = 2, 32; P < 0.001).

^fThere was a significant difference between the treatments of 1°- and 2°-form bacteria or freeze-killed control (F = 67.5, df = 2, 67; P < 0.0001).

^gNA, not applicable.