TABLE 1.
Specific activities of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase on pyrocatechol in cell extracts of wild-type R. pyridinivorans PAa
| Growth substrate | Protein concn in cell extract (mg/ml) | Sp act (nmol/min/mg of protein) ofa:
|
|
|---|---|---|---|
| Catechol 1,2-dioxygenase | Catechol 2,3-dioxygenase | ||
| Acetate | 0.9 | 31 | NDb |
| Benzoic acid | 4.7 | 870 | ND |
| Nutrient broth | 0.61 | 29 | ND |
| TSB | 2.7 | 7.9 | ND |
| OBT | 0.3 | ND | 0.48 |
a
The values are the means of at least three determinations, all of which gave very similar activities.
b
ND, no detectable activity.