TABLE 2.

Oligonucleotides used in the study

Oligonucleotide	Nucleotide sequence (5′→3′)a	Useb
p1	CGATCCTGCTTTAACTGCTGTTGAAACTGGTGCTACTAT	VP1
p2	CGATAGTAGCACCAGTTTCAACAGCAGTTAAAGCAGGAT	VP1
p3	CTGAGAATTCAGTTACAGCAACCAACG	PCR
p4	TTTAAAGCTTGTTTTTCCTAACAAAGGCC	PCR
p5	CCATGGTACAATCAAGTTTAAAGAAATCTC	PCR
p6	CCTGCTTTAACTGCTGTTGAAACTGGTGCTACTGCCGCCGATCAAACTGCTC	R-PCR, site II/VP1
p7	AGTAGCACCAGTTTCAACAGCAGTTAAAGCAGGCTTATCGCTTACCTTAGAACCTG	R-PCR, site II/VP1
p8	CCTGCTTTAACTGCTGTTGAAACTGGTGCTACTAATGATAAGGTTGCAGCTAACG	R-PCR, site III/VP1
p9	AGTAGCACCAGTTTCAACAGCAGTTAAAGCAGGAGCATCAGCTGTAGTCAATGC	R-PCR, site III/VP1
p10	TACCGAATTCGGGACAGGTGCTAGAGAC	PCR
p11	AGTAGCACCAGTTTCAACAGCAGTTAAAGCAGGAGCCATAGTAGCCTTAGAAG	R-PCR, site IV/VP1
p12	CCTGCTTTAACTGCTGTTGAAACTGGTGCTACTAAGTTAGCTTCTTCAAAGAGTC	R-PCR, site IV/VP1
p13	GTATGAATTCGAAATGACTTCAGAAAAGG	PCR
p14	GACAGGATCCATATAGAAGAAAAGGGC	PCR
p15	CACAAAGCTTTGAAGAAGCAGCACTGTCC	PCR
p16	CCAAGATCTAGTCTTATAACTATACTG	PCR
p17	CAGATCCTCTTCTGAGATGAGTTTTTGTTCCTTATCGCTTACCTTAGAACCTG	R-PCR, site II/c-Myc
p18	GAACAAAAACTCATCTCAGAAGAGGATCTGGCCGCCGATCAAACTGCTC	R-PCR, site II/c-Myc
p19	GTTCGGTACCATCAACAAAGCTCACCTA	PCR
p20	CTGAGGTACCTCTCTTTACTTGGGCCTTGC	PCR
p21	CAGATCCTCTTCTGAGATGAG	PCR
p22	TCATCTCAGAAGAGGATCTG	PCR
p23	ACTATCCCGACCGCCTTAC	PCR
p24	CGTAACTGCCATTGAAATAGACC	PCR
p25	GAGATAAGAATTGTTCAAAGCTA	PCR
p26	TGCAGGTACCGATTACAAAGGCTTTAAGC	PCR
P27	CATGGGATCCCTGGTACACAAGTACTTGG	PCR
p28	ATTTGAATTCAGCAGCATAGACTGTTGAG	PCR

^aThe epitope-encoding nucleotides are underlined.

^bR-PCR, recombinant PCR; VP1, a poliovirus type 3 VP1 epitope; c-Myc, an epitope from the human c-myc proto-oncogene; sites II to IV refer to the insertion sites of the epitope-encoding nucleotides in the slpA gene.