TABLE 2.

Growth and kinetic parameters of VC-assimilating bacteria

Strain	Growth yielda (g of protein/mol of substrate)		Maximum specific growth rate (day−1)		k (nmol/min/mg of protein)		Ks (μM)		Epoxyethane production from ETH (nmol/min/mg of protein)
	VC	ETH	VC	ETH	VC	ETH	VC	ETH	VC-grown	Acetate-grown
Mycobacterium
JS60	6.6 ± 0.7	11.2 ± 0.6	0.22 ± 0.02	0.58 ± 0.05	9.7 ± 0.2	25.4 ± 0.8	0.5 ± 0.1	0.9 ± 0.1	12.1 ± 3.7	0
JS61	6.2 ± 1.5	12.1 ± 1.6	0.21 ± 0.02	0.76 ± 0.07	9.0 ± 0.2	43.5 ± 1.4	0.8 ± 0.2	4.0 ± 0.2	8.0 ± 2.8	0
JS616	5.4 ± 1.1	12.8 ± 1.1	0.17 ± 0.02	0.39 ± 0.02	15.4 ± 0.6	20.4 ± 1.0	3.2 ± 0.3	4.0 ± 0.5	13.9 ± 9.3	0
JS617	6.0 ± 0.6	11.1 ± 0.5	0.23 ± 0.02	0.64 ± 0.05	16.0 ± 0.4	30.6 ± 1.4	0.8 ± 0.1	1.5 ± 0.1	6.7 ± 0.7	0
Nocardioides sp. strain JS614	10.3 ± 0.8	21.2 ± 1.8	0.71 ± 0.04	0.91 ± 0.08	43.1 ± 4.2	21.5 ± 1.6	1.2 ± 0.2	1.0 ± 0.2	22.0 ± 4.1	0
Mycobacterium sp. strain L1b	7.6	11.9	0.96	NRc	100	NR	3.2	NR	NR	NR
Pseudomonas sp. strain DL1b	7.2 ± 0.3	13.1 ± 1.7	0.046	0.071	4.6 ± 0.2	3.8 ± 0.1	1.2 ± 0.2	18.8 ± 1.2	NR	NR

^aAll data from the present study are averages of three replicates; error values represent 95% confidence intervals.

^bData for strains L1 (25) and DL1 (58) were converted to units of protein, assuming a value of 55% protein in dry weight.

^cNR, not reported.