FIG. 2.

Southern blot analysis of wild-type R. eutropha and R. eutropha SS14. Genomic DNA from the wild type and integrant SS14 were extracted and digested with SacI, PstI, EcoRI, and RsrII. A 400-bp phaP promoter sequence (the FspI/SacI fragment of plasmid pUCPPCm, shown in grey) was used to probe the blot. (A) Results of Southern blot analysis of genomic DNA of the wild type and the integrant probed with a horseradish peroxidase-labeled phaP promoter. The sizes of the markers (base pairs) are indicated on the left. (B) Map of the analyzed genomic region in the wild type and the integrant, indicating the probe and the various restriction enzymes used. S, SacI; E, EcoRI; P, PstI; R, RsrII. The open reading frames of OPH and GA24 (phaP) are also indicated.