TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| S. mutans strains | ||
| GS-5 | Erys, Kans, serotype c human isolate | SUNYaBa |
| CA1101 | Erys; GS-5::pAYCA1101; ComA− | This study |
| CC1301 | Eryr; GS-5::pAYCC1301; ComC− | This study |
| CD1401 | Eryr; Cmr, GS-5::pAYCD1401; ComD− | This study |
| CE1501 | Eryr; GS-5::pAYCE1501; ComE− | This study |
| GS-5(gtfB)::pSIV2 | Eryr; GS-5::pSIV2 alone | 2 |
| GS-5(gtfB)::pSIV2-SGPAN | Eryr; strain GS-5 expressing antisense sgp RNA from the host chromosome | 2 |
| TnSp-1 | Sptr, Tetr, GS-5 with dgk insertion | Lis and Kuramitsu (SUNYaB) |
| AYRA1 | Eryr; GS-5::pAYRA1; RelA-SpoT− | This study |
| luxS | Eryr, GS-5 with luxS insertion | W. Shi (UCLA)b |
| E. coli DH5α | Cloning host | GIBCO BRL |
| Plasmids | ||
| pResEmMCS10 | Emr; integration vector | 41 |
| pMCL200 | Cmr, cloning vector | 31 |
| pAYCA1101 | Emr, pResEmMCS10 bearing comA upstream and downstream regions | This study |
| pAYCC1301 | Emr, pResEmMCS10 bearing comC upstream and downstream regions | This study |
| pAYCC1302 | Emr, pResEmMCS10 bearing comC upstream regions | This study |
| pAYCD1401 | Cmr, Emr, pMCL200 bearing Emr cassette, comD upstream and downstream regions | This study |
| pAYCE1501 | Emr, pResEmMCS10 bearing comE upstream and downstream regions | This study |
| pSIV2 | Emr; integration vector bearing gtfB 1.083-kb EcoRV fragment | 2 |
| pSIV2-SGPAN | Emr; pSIV2 bearing the sgp gene in antisense orientation downstream of the scrB promoter | 2 |
| pAYRA1 | Emr, pResEmMCS10 bearing relA-spoT 640-bp internal fragment | This study |
a
SUNYaB, the culture collection in Department of Oral Biology, State University of New York, Buffalo, N.Y.
b
UCLA, University of California at Los Angeles.