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. 2026 May 19;13:1146. doi: 10.1038/s41597-026-07299-9

Table 2.

BSA Dataset Field Descriptions.

Field Applies To Description
NCBI tax id

⊠ Bacteria

□ Virus

A stable, unique numerical identifier that corresponds to a bacteria node in the taxonomic tree.
Virus GENBANK accession

□ Bacteria

⊠ Virus

A unique, stable alphanumeric identifier assigned to a viral GenBank sequence record.
Kingdom

⊠ Bacteria

⊠ Virus

Bacteria (“B”) or Virus (“V”).
Bacterial Classification

⊠ Bacteria

□ Virus

Gram-positive (“G+”), Gram-negative (“G-”) or Acid Fast (“AF”).
Spore

⊠ Bacteria

□ Virus

Spore forming (“Y” for yes) or non-spore forming (“N” for no) bacteria.
Acceptable Containment Level

⊠ Bacteria

⊠ Virus

For bacteria, BSL comes from BacDive data. Viral values are assigned based on Biosafety in Microbiological and Biomedical Laboratories (BMBL): https://www.cdc.gov/labs/media/pdfs/2025/08/SF__19a_308133-A_BMBL6_00-BOOK-WEB-final-3.pdf or based on the NIH Guidelines for Research or based on Risk Group values from NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules (NIH Guidelines) - November 2013. Note the assumption that BSL = Risk Group.
Genome Type

□ Bacteria

⊠ Virus

Viral genome type, either DNA or RNA.
Ultrastructure

□ Bacteria

⊠ Virus

Viral ultrastructure, enveloped (env), non-enveloped (non-env), quasi-enveloped (quasi-env), or not applicable (NA). SMEs used ICTV to inform value assignments to viruses based on phylum or family.
Regulatory Strain / Species

⊠ Bacteria

⊠ Virus

EPA OCSPP 810 guideline regulatory strains (“Y” for yes or “N” for no).
Phyla

⊠ Bacteria

⊠ Virus

Phyla are entered or selected from the dropdown menu.
Order

⊠ Bacteria

⊠ Virus

Order is entered or selected from the dropdown menu.
Family

⊠ Bacteria

⊠ Virus

Family is entered or selected from the dropdown menu.
Genus

⊠ Bacteria

⊠ Virus

Genus is entered or selected from the dropdown menu.
Aerobic

⊠ Bacteria

□ Virus

Aerobic or facultative anaerobic (“Y” for yes) or microaerophilic or obligate anaerobic (“N” for no).
Single stranded / Double stranded

□ Bacteria

⊠ Virus

Viral genome material, single stranded (“ss”) or double stranded (“ds”).
Motility

⊠ Bacteria

⊠ Virus

Motile (“Y” for yes) or non-motile (“N” for no) bacteria.
Risk Group

□ Bacteria

□ Virus

Values from NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules (NIH Guidelines) - November 2013.
Select Agent

⊠ Bacteria

⊠ Virus

Based on Select Agents and Toxins List https://selectagents.gov/ from May 29, 2024. (“Y” for yes or “N” for no).
Notes

⊠

⊠ Bacteria

⊠ Virus

Mainly used to explain BSL value assignments made. Can be used to detail other value assignments.
Source

⊠ Bacteria

⊠ Virus

Bacteria from BacDive: https://bacdive.dsmz.de/advsearch

Viruses from ICTV: https://ictv.global/sites/default/files/VMR/VMR_MSL39.v1_20240912.xlsx

SME Additions: blank