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GRE formate lyase structure and mechanism. (A) Summary of the proposed PFL mechanism. (B) Pyruvate formate lyase structure (PDB ID: 1H16) showing pyruvate (PYR) binding site. Distances from Gly734 to Cys419 to Cys418 to PYR–C2 are shown. (C) Pyruvate formate lyase structure showing CoA binding site near predicted substrate channel.
