Figure 2.

Ct-RSF and hrp45 are coimmunoprecipitated. Nuclear extract from C. tentans cells was immunoprecipitated with anti-hrp45 antibodies (A) or anti-Ct-RSF antibodies (B). Ct-RSF coimmunoprecipitated with hrp45 and vice versa (A and B, lanes 2). The hrp45 signal in B (arrows) is located just below the position of the antibody used in the experiment. Controls represent immunoprecipitations in the absence of specific antibodies. RNase treatment of the nuclear extracts before immunoprecipitation did not affect the signal (A and B, lanes 5).