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Ct-RSF represses formation of spliceosomal E complex. HeLa cell nuclear extract was depleted for ATP and incubated with labeled AdML pre-mRNA at 30°C for the indicated times. Complexes were analyzed on 1.5% agarose gels. Lanes 3 and 4, increasing amounts (1 and 1.5 μg) of Ct-RSF repressed E complex formation. Positions of E and H complexes are shown to the right.
