Table 2.
Genes required for growth under anaerobic conditions
| Functional class | ORF | Gene | Function |
|---|---|---|---|
| Amino acid metabolism | YGL148 | ARO2 | Chorismate synthase; required for biosynthesis of aromatic amino acids |
| YPR060 | ARO7 | Chorismate mutase; required for biosynthesis of tyrosine and phenylalanine | |
| YJR139 | HOM6 | Homoserine dehydrogenase; required for biosynthesis of methionine and threonine | |
| YOR184 | SER1 | Phosphoserine transaminase; required for biosynthesis of l-serine | |
| YPR074 | TKL1 | Transketolase I; required for the pentose phosphate pathway and for biosynthesis of aromatic amino acids | |
| Fermentation of glucose and metabolism of cofactors | YOL086 | ADH1 | Alcohol dehydrogenase; required for glucose fermentation and catabolism of tryptophan and phenylalanine |
| YOR209 | NPT1 | Nicotinate phosphoribosyltransferase; required for salvage pathway of NAD+ biosynthesis, affects silencing | |
| YBL033 | RIB1 | GTP cyclohydrolase II; required for riboflavin, FMN, and FAD biosynthesis | |
| YOL143 | RIB4 | Riboflavine synthase; required for riboflavin, FMN, and FAD biosynthesis | |
| Regulation of transcription and translation | YKL139 | CTK1 | RNA polymerase II C-terminal domain kinase |
| YML112 | CTK3 | RNA polymerase II C-terminal domain kinase | |
| YNL139 | RLR1 | RNA polymerase II transcription elongation, mRNA export | |
| YBL093 | ROX3 | RNA polymerase II holoenzyme component | |
| YGL135 | RPL1B | Structural constituent of the large (60S) ribosomal subunit | |
| Vesicular transport | YAL026 | DRS2 | P-type ATPase, potential aminophospholipid translocase |
| YNL243 | SLA2 | Protein involved in membrane cytoskeleton assembly, required for cell polarization and endocytosis | |
| YLR240 | VPS34 | Phosphatidylinositol 3-kinase, required for vacuolar protein sorting | |
| Protein kinases | YLR226 | BUR2 | Cyclin-dependent protein kinase regulator |
| YPL031 | PHO85 | Cyclin-dependent protein kinase, regulates glycogen and phosphate metabolism | |
| YKL126 | YPK1 | Serine/threonine protein kinase required for endocytosis | |
| Mitochondrial function and biogenesis | YLR304 | ACO1 | Aconitase; required for TCA cycle, mutation leads to glutamate auxotrophy |
| YBL099 | ATP1 | α-Subunit of F1 sector of F1F0 ATP synthase | |
| YJR121 | ATP2 | β-Subunit of F1 sector of F1F0 ATP synthase | |
| YBR039 | ATP3 | γ-Subunit of F1 sector of F1F0 ATP synthase | |
| YNL315 | ATP11 | Molecular chaperone required for assembly of F1 sector of the F1F0 ATP synthase | |
| YJL180 | ATP12 | Molecular chaperone required for assembly of F1 sector of the F1F0 ATP synthase | |
| YOR125 | CAT5 | May encode a protein involved in one or more mono-oxygenase or hydroxylase steps of ubiquinone biosynthesis | |
| YJR144 | MGM101 | Involved in mitochondrial genome maintenance | |
| YLR369 | SSQ1 | Chaperon involved in the synthesis and assembly of iron/sulfur clusters into proteins | |
| YDR470 | UGO1 | Outer membrane protein required for mitochondrial fusion | |
| YPR024 | YME1 | Mitochondrial inner membrane peptidase | |
| Miscellaneous | YGL084 | GUP1 | Multimembrane-spanning protein and putative glycerol transporter that is essential for proton symport of glycerol |
| YDL116 | NUP84 | Subunit of the nuclear pore complex | |
| YBL031 | SHE1 | Cytoskeletal protein of unknown function; overexpression causes growth arrest | |
| Unknown function | YBL100 | Dubious open reading frame | |
| YJR120 | Hypothetical open reading frame | ||
| YLR202 | Dubious open reading frame |