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Scd5p constitutively undergoes nuclear-cytoplasmic shuttling via a Crm1p-dependent pathway. GFP-Scd5p was expressed in wild-type CRM1 (SL4619), or crm1-1 (SL4620) strains. Cells were grown at 25°C, shifted to 25 or 37°C for 30 min, and examined by fluorescence microscopy. DAPI staining was used to localize nuclear DNA.
