Table 1.
Strains used in this study
| Strain No.a | Genotype |
|---|---|
| SL3004 | MATα leu2 ura3 trp1-901 his3 ade2-101 lys2-801 gal4 gal80 |
| SL3799 | MATa ark1Δ:HIS3 prk1Δ:URA3 ura3-52 his3-Δ200 leu2-3,112 lys2-801 |
| SL4397 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pJSC3[CEN, LEU2, scd5-ΔCT] |
| SL4403 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pKRH24[CEN, LEU2, scd5-Δ3R] |
| SL4418 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pCC545[CEN, LEU2, SCD5] |
| SL4436 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pCC545[CEN, LEU2, SCD5] |
| SL4619 | MATα leu2 ade2-1 ura3-1 trp1-1 can1-100 xpo1Δ::LEU2(crm1Δ) pKW440[CEN, HIS3, XPO1(CRM1)] |
| SL4620 | MATα leu2 ade2-1 ura3-1 trp1-1 can1-100 xpo1Δ::LEU2(crm1Δ) pKW457[CEN, HIS3, xpo1-1(crm1-1)] |
| SL4642 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC36[CEN, LEU2, GFP-scd5-ΔCT] |
| SL4645 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC35[CEN, LEU2, GFP-scd5-Δ3R] |
| SL4675 | MATa leu2 ura3-52 trp1 his3-Δ200 pJSC42[CEN, LEU2, GFP-CT] |
| SL4683 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC46[CEN, LEU2, GFP-scd5Δ9R] |
| SL4687 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC41[CEN, LEU2, GFP-scd5-ΔNLS] |
| SL4702 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC31[CEN, LEU2, GFP-SCD5] |
| SL4704 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pJSC45[CEN, LEU2, scd5-Δ9R] |
| SL4706 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pCC545[CEN, LEU2, SCD5] |
| SL4708 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pKRH24[CEN, LEU2, scd5-Δ3R] |
| SL4709 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC45[CEN, LEU2, scd5-Δ9R] |
| SL4714 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC49[CEN, LEU2, GFP-scd5-Δ33] |
| SL4725 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC53[CEN, LEU2, GFP-scd5LLL>AAA] |
| SL4726 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC52[CEN, LEU2, scd5LLL>AAA] |
| SL4727 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pJSC52[CEN, LEU2, scd5LLL>AAA] |
| SL4738 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pKRH24[CEN, LEU2, scd5-Δ3R] |
| SL4741 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pJSC45[CEN, LEU2, scd5-Δ9R] |
| SL4751 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC3[CEN, LEU2, scd5-ΔCT] |
| SL4759 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pJSC52[CEN, LEU2, scd5LLL>AAA] |
| SL4764 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pJSC3[CEN, LEU2, scd5-ΔCT] |
| SL4770 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC57[CEN, LEU2, scd5-ΔCT+NES] |
| SL4772 | MATa/MATα leu2/leu2 ura3-52/ura3-52 trp1/trp1 his3-Δ200/his3-Δ200 scd5Δ::TRP1/scd5Δ::TRP1 pJSC57[CEN, LEU2, scd5-ΔCT+NES] |
| SL4774 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC59[CEN, LEU2, GFP-scd5-ΔCT+NES] |
| SL4784 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pJSC57[CEN, LEU2, scd5-ΔCT+NES] |
| SL5143 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC34[CEN, URA3, GFP-SCD5] pTMN2[CEN, LEU2, GAL:GST-CT] |
| SL5151 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 pJSC34[CEN, URA3, GFP-SCD5] pTB338[CEN, LEU2, GAL:GST] |
| SL5227 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pOTT4[CEN, LEU2, scd5-ΔNLS] |
| SL5228 | MATa leu2 ura3-52 trp1 his3-Δ200 scd5Δ::TRP1 bar1Δ::HIS3-MX4 pOTT6[CEN, LEU2, SCD5] |
| SL5231 | MATa bar1-1 his3-Δ200 leu2 ura3-52 pTB338[CEN, LEU2, GAL:GST] |
| SL5232 | MATa bar1-1 his3-Δ200 leu2 ura3-52 pTMN2[CEN, LEU2, GAL:GST-CT] |
| YPJ96—4A | MATa trp1-901 leu2-3,112 ura3-52 his3-Δ200 gal4Δ ade2 gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ |
a
All strains are from this laboratory except for SL3799 (David Drubin), SL4619 and SL4620 (Alan Tartakoff), and YPJ96-4A (Elizabeth Craig).