Figure 3.

Identification of the Src domains involved in the interaction with the Na⁺/K⁺-ATPase. (A) Schematic presentation of structures of Src. (B) Coomassie blue staining of GST-Src, GST-SH2, GST-SH3, GST-SH3SH2, and GST-kinase. (C) Binding of GST-Src, GST-SH3SH2, GST-kinase, GST-SH2, but not GST-SH3, domains to the Na⁺/K⁺-ATPase. An aliquot (2 μg) of the purified Na⁺/K⁺-ATPase was used for each binding assay. The same experiments were repeated three times.