Figure 1.

Maturation of V2R-V206D upon treatment with chemical chaperones. Confluent V2R-V206D- or V2R-S167T-expressing MDCK cells were untreated, incubated for 16 h with 4% glycerol, 1% DMSO, 1% TMAO, 5 mM 4-PBA or at 27°C, or treated for 2 h with 1 μM thapsigargin, lysed in Laemmli sample buffer, loaded on a 10% polyacrylamide gel, and subjected to immunoblotting. V2R-V206D or -S167T was detected using anti-GFP antibodies (top and middle, respectively). To ensure equal loading of the samples, blots were incubated with β-actin antibodies (bottom). Duplicate samples are shown. Mass indications in kilodaltons are given on the left.