Figure 3.

The expression of molecular chaperones is not affected by osmolyte treatment. MDCK-wtV2R or -V2R-V206D cells were left untreated or incubated for 16 h with 4% glycerol, 1% DMSO, or 1% TMAO, or 2 h with 1 μM thapsigargin or 1 μM curcumin (indicated), lysed, and subjected to immunoblotting as described in the legend of Figure 1. As primary antibodies, rabbit-anti-GFP (top), rabbit-anti-calnexin (CNX), mouse-anti-HSP70 (HSP70), or mouse-anti-HSP90 (HSP90) were used. Per lane, 10 μg of total cell lysate was loaded.