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. 2006 Jan;17(1):379–386. doi: 10.1091/mbc.E05-06-0579

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Copyright © 2006, The American Society for Cell Biology

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Figure 5. — V2R-V206L, -V206E, and -V206R are expressed as mature proteins. (A) MDCK cell lines stably expressing either wild-type V2R, V2R-V206L, -V206E, -V206R, or -V206D (indicated) were grown to confluence, lysed, and analyzed by immunoblotting for GFP. The masses of the immature (60-kDa) and mature (75-kDa) V2R proteins are indicated on the left. (B) MDCK cells stably expressing the GFP-tagged V2R-V206E were grown to confluence, fixed, immunocytochemically stained using anti-PDI and anti-E-cadherin antibodies, and analyzed by CLSM as described in the legend of Figure 2. Bar, 10 μm.