Figure 5.

DTT stress induces protein aggregation. Induction of DTT resistance by oxidative stress. Wild-type (A) and tsa1 (B) mutant cells grown in YEPD media were pretreated with 50 μM H₂ O₂ for 1 h before growth in the presence of 2 or 4 mM DTT. Growth was monitored by absorbance at 600 nm. (C) Aggregation of ribosomal proteins in the tsa1 mutant and in response to DTT. Insoluble proteins were extracted from the wild-type and tsa1 mutant strains grown in YEPD media after treatment with 16 DTT or 4 mM H₂ O₂ for 1 h. Proteins were separated by SDS-PAGE and detected by silver staining. The indicated proteins were identified via MALDI-TOF mass spectrometry.