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CREB4 localizes to the ER and Golgi dependent on the putative transmembrane domain. Top, schematic summary of CREB4 and variants. Bottom, cells were transfected with were transfected with 2 μg of the appropriate expression vector as indicated and processed as in Materials and Methods. Removal of the TM domain in the context of the full-length protein, or deletion of the TM domain and the C-terminal region, resulted in compete relocation of the protein to the nucleus.
