Figure 2.

Physical interaction between HDAC5 and ANKRA2. (A) 293T cells were cotransfected with expression vectors encoding FLAG-tagged HDAC5 and Myc-ANKRA2. Cell extracts were immunoprecipitated (IP) with anti-FLAG antibodies and proteins in the immune complexes were analyzed by Western blotting with the indicated antibodies (Blot). Crude lysates were analyzed by Western blotting to control for variability in protein expression (Input). (B) 293T cells were transfected with expression vectors encoding FLAG-HDAC5 and several deletion mutants of Myc-ANKRA2 (shown in C). Cell extracts were immunoprecipitated (IP) with anti-FLAG antibodies and proteins were analyzed by Western blotting as described above. (C) Shown are schematic depictions of the ANKRA2 deletion mutants used in B and a summary of the coimmunoprecipitation results.