Figure 4.

Expression of class II HDACs in macrophages. (A) The carboxy-terminal ankyrin repeats of ANKRA2 are highly homologous to those of RFXANK. Shown is the percentage of amino acid identity between the four ankyrin repeats of each protein. The amino-termini of ANRKA2 and RFXANK are distinct, with ANKRA2 containing a helix-loop domain and RFXANK, a prolinerich PEST domain. (B) RT-PCR for HDAC4 and HDAC5 mRNA transcripts was performed using total RNA extracted from mouse primary macrophages obtained from peritoneal or bone marrow or the J774 macrophage cell line. cDNAs synthesized from total RNA obtained from brain or heart of HDAC4- or HDAC5-null mice, respectively, was used to control for PCR primer specificity.