Figure 2.

Grn1 is required for wild-type growth and encodes a nucleolar protein. (A) Grn1 (SPBC26H8.08c) was deleted as described in the text. Tetrad dissection yielded two fast-growing (Wild type) and two slow-growing (null mutant) colonies. (B) A null mutant expressing full-length Grn1p:GFP (YNB544) or an empty vector (YNB546) were in EMM-leu medium. Optical density (OD₅₉₅) was determined at the indicated time points. (C) YNB544 (see above) was used to show the localization of Grn1p. Nuclear DNA was stained with DAPI. Nucleoli were revealed by indirect immunostaining with anti-Fibrillarin (AbCam, Cambridge, United Kingdom). Independent or merged images are indicated. (D) Wild-type (YNB483) and null mutant (YNB484) strains were stained with DAPI and Aniline blue for visualizing the nucleus and septum, respectively. Arrows indicate the septum. Bar, 10 μm.