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. 2006 Jan;17(1):460–474. doi: 10.1091/mbc.E05-09-0848

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Figure 7. — Expression of a human gene GNL3L rescues the growth defect of the null mutant. (A) Yeast strains expressing the indicated proteins transcribed from an nmt1 promoter were tested for growth in EMM-leu medium in the presence (nmt1 OFF) and absence (nmt1 ON) of thiamine. (B) Growth of GNL3l:FLAG (YNB858) and Grn1:FLAG (YNB859) is compared with a null mutant (YNB484) in YES medium. (C) GNL3L and Grn1p colocalize with nucleolin in Cos-7 cells. Localization of GNL3L and Grn1p was determined by confocal microscopy. Nucleoli were revealed by immunostaining with anti-nucleolin. (D) S. pombe cells showing localization of GNL3L:GFP in S. pombe. Wild-type S. pombe was transformed with an expression vector containing GNL3L:FLAG (BNB395). Nucleoli were revealed by immunostaining with anti-fibrillarin and GNL3L with anti-FLAG. Independent or merged images are indicated. Bar, 4 μm.