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siRNA knockdown of GNL3L in HeLa cells. (A) Cultures of HeLa cells were transfected with the indicated siRNA sequences. After a 24-h transfection, the siRNA expression cells were selected in the presence of neomycin (500 μg/ml) for 120 h and photographed. (B) RT-PCR analysis of GNL3L transcript. Total RNA was isolated from the cells transfected with the indicated siRNA. RT-PCR analysis was performed as described in Materials and Methods. β-actin was used as internal control.
