Figure 1.

Rtt107 physically interacts with Slx4 and Slx1. (A) Extracts from yeast strains expressing the indicated epitope-tagged proteins were immunoprecipitated with IgG agarose or α-VSV followed by protein G agarose. Ten percent of the input extract (E) and the entire immunoprecipitate (IP) were fractionated by SDS-PAGE. Immunoblots were probed with α-VSV to detect Rtt107-VSV, peroxidase-anti-peroxidase to detect Slx4-TAP. (B) Extracts were treated as in A, and immunoblots were probed with α-HA to detect Slx4-HA and Slx1-HA or rabbit IgG to detect Rtt107-TAP.