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. 2006 Jan 23;34(2):606–612. doi: 10.1093/nar/gkj453

Figure 2.

Figure 2

Induction of a DNA loop in vitro. The fraction ([Of]/[Ot]) of free DNA fragment (total fragment concentration is 5 × 10−11 M) in the presence of 2 × 10−10 M wt Lac repressor is plotted as a function of IPTG concentration. Note the different scale compared with Figure 1. (A) Loop complex of wt Lac repressor with a DNA fragment carrying two lac operators separated by 16 helical turns. (B) Control: complex of wt Lac repressor with a DNA fragment carrying a single lac operator. To normalize the curves, values at 600 µM IPTG are set to 1.