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. 2006 Jan 20;34(2):528–542. doi: 10.1093/nar/gkj461

Figure 3.

Figure 3

(A) Structure and GC-content of the chromosomal region on human chromosome 22q11.2 that spans the catechol-o-methyltransferase gene (COMT), the thioredoxin reductase 2 gene (TXNRD2) and the armadillo repeat gene deleted in VCFS (ARVCF). Vertical black bars represent exons. (B) To determine the methylation profile of the 100 kb TXNRD2-COMT-ARVCF region, 384 oligonucleotides (50mers, black horizontal bars) were designed based on the restriction sites for the methylation-sensitive endonucleases, HpaII, Hin6I and AciI (additional alternative enzymes are HpyCH4IV or Hin1I). Depending on the methylation status of the CpG-dinucleotides several combinations of amplicons (grey horizontal bars) can potentially hybridize to the oligonucleotides. (C) Typical hybridization patterns of the hypomethylated fraction of human gDNA on the COMT oligonucleotide-microarray. As discussed in Results, the complexity and informativeness of the hybridization signals increases with increasing number of methylation-sensitive restriction enzymes.