Figure 5.

Loss of heterozygosity analysis in laser capture microdissected ovarian malignancies. Normal stroma and malignant cell populations were enriched using laser capture microdissection for cancer specimens heterozygous for a SNP in exon 16 of M6P/IGF2R to assess for potential allelic loss. Peripheral blood lymphocytes were available for analysis in one case (number 40). Nucleotide sequencing was followed by PhosphorImager analysis to quantify each allele, shown as the percent of the C allele present for this C/T polymorphism. Loss of heterozygosity, defined by an allelic imbalance >4:1 in the malignant cells, is evident in three of the four samples analyzed.