Figure 2.

The PNA sensor probe differentiated the 12 possible K-ras mutations in codons 12 and 13 from the wild-type. Mutant K-ras were generated by PCR-mediated site-directed mutagenesis and were used as PCR templates to test the resolution of the PNA sensor probe. All the PCR were performed under non-clamping conditions, using primer F1, and 72°C for extension. Melting curves (A) and melting peaks (B) were plotted after PCR. Filled and open arrowheads indicate wild-type and mutant melting peaks, respectively. Mut, mutant; WT, wild-type; NTC, no template control.