Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Extension temperature affected the efficiency of the PNA-mediated PCR clamp. PCR were performed using either 100 ng wild-type (WT), 1 ng mutant (Mut) or a mix containing 100 ng wild-type and 1 ng mutant genomic DNA (WT + Mut) as templates and F1 as the forward primer. Extension temperatures were 72 (A), 65 (B) or 60°C (C). Filled and open arrowheads indicate wild-type and mutant melting peaks, respectively.
