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Assay sensitivity for detection of K-ras mutants in a large excess of wild-type DNA. 100 ng wild-type genomic DNA plus various amounts of mutant genomic DNA were used as templates for clamp-PCR. Under the optimal condition using F2 as the forward primer and 60°C as extension temperature, the assay detected the signal from as few as 10 pg mutant DNA without interference from the wild-type DNA. Filled and open arrowheads indicate wild-type and mutant melting peaks, respectively.
