Table 1. TnAraOut V. cholerae mutants with Dictyostelium-attenuated phenotype.
| Strain | Gene | VC number | Distance from ATG (gene length) | Homology | blastp* (bits/E value) | Function ascribed to homologue |
|---|---|---|---|---|---|---|
| SP17 | vasA | VCA0110 | +510 (1,770) | RL impG | 242/3e-62 | Impaired Rhizobium plant infection |
| SP65 | vasA | VCA0110 | +704 (1,770) | RL impG | 242/3e-62 | See above |
| SP44 | vasH | VCA0117 | +96 (1,593) | EC rtcR | 133/1e-31 | Sigma-54 dependent activator in E. coli |
| SP95 | vasK | VCA0120 | +1477 (3,546) | LP icmF | 154/6e-38 | Type IV protein secretion in L. pneumophila |
| SP7 | vgrG-2 | VCA0018 | +985 (2,085) | EC vgrG | 417/8e-117 | Homologous to VgrG-1 that catalyzes action-crosslinking in eukaryotic cells |
| SP83 | vgrG-2 | VCA0018 | +704 (2,085) | EC vgrG | 417/8e-117 | See above |
| SP109 | rpoN | VC2529 | +338 (1,464) | VC rpoN | 862/0.0 | Alternative σ54 subunit of RNA polymerase |
RL, R. Ieguminosarum; EC, E. coli; LP, L. pneumophila; VC, V. cholerae.
*
Position-specific iterated and pattern-hit initiated blast (psi- and phi-blast) statistics. Bits, normalized raw alignment score; E value, expectation value.