Table 1. Summary data for mitochondrial and nuclear genotypes of rescued A549 ρ° clones.
| Mitochondrial genome
|
Nuclear genome
|
|||||||
|---|---|---|---|---|---|---|---|---|
| RFLP
|
SNP
|
Col1A2
|
STR
|
|||||
| Donor cells | A549 | Donor | A549 | Donor | A549 | Donor | A549 | Donor |
| d198 | 18/24 | 21/24 | 0/24 | 21/24 | 14/14 | 0/14 | 3/3 | 0/3 |
| d242 | 0/24 | 24/24 | 0/31 | 31/31 | 10/10 | 0/10 | 3/3 | 0/3 |
| d235 | 0/24 | 24/24 | 0/24 | 24/24 | 6/6 | 0/6 | 3/3 | 0/3 |
| fibro | 0/24 | 24/24 | 0/23 | 23/23 | N/A | N/A | 3/3 | 0/3 |
The mitochondrial genotype was assayed by RFLPs and SNP. Nuclear genotypes of rescued clones from several coculture experiments were assayed by a polymorphism in the intron downstream of exon 28 in the type 1 collagen (COL1A2 exon 28 IVS + 15) and by analysis of nine different microsatellite markers on different chromosomes (STR). The observed mitochondria genotypes indicate transfer of mitochondria from three different hMSC donors (d198, d242, and d235) and one fibroblast donor (fibro). The nuclear genotypes of rescued clones indicate that in all cases the nucleus was derived from the original A549 cell line, excluding cell fusion as the primary mechanism for mitochondria transfer. N/A, not assayed.