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pSUPER-AGRPi construct decreases AGRP immunoreactivity. (a) Overall design of the pSUPER-RNAI plasmid construct targeted against AGRP and the predicted transcript with the hairpin loop. (b and c) Immunocytochemistry of fasted mice transfected with the (b) control pSUPER-GFPi vector compared to (c) mice transfected with the pSUPER-AGRPi vector shows decreased AGRP immunoreactivity with the RNAI plasmid construct targeted to AGRP.
