Table 1.
EF-hand-containing proteins (excluding CPKs and CRKs) in Arabidopsis
| ID number§ | Size (amino acids) | Number of EF hands | Domains*/remarks† | Targeting‡ | Published name | References |
| Group I | ||||||
| At1g02150 | 638 | 1 | PPR repeats, NLS | sc (.616) | ||
| At1g06220 | 987 | 2 | Elongation factor aEF-2 | |||
| At5g25230 | 973 | 1 | ATP_GTP_A, EFG_C, GTP_EFTU(_D2) | |||
| At1g17090 | 93 | 1 | - | sc (.944) | ||
| At1g55040 | 849 | 1 | Zn-finger in Ranbp and others | ch (.823) | ||
| At1g74430 | 271 | 1 | Myb DNA-binding domain | |||
| At2g03150 | 1276 | 1 | - | mt (.870) | ||
| At5g40690 | 210 | 1 | - | mt (.656) | ||
| At3g13500 | 110 | 1 | - | |||
| At1g69030 | 320 | 1 | - | |||
| At3g59820 | 755 | 1 | - | mt (.666) | ||
| At1g73440 | 254 | 2 | Josephin, UIM | ch (.941) | ||
| At3g07740 | 548 | 1 | Zinc finger, Myb DNA-binding, GatC | |||
| At5g06260 | 424 | 1 | - | |||
| At4g00140 | 257 | 1 | - | ch (.792) | ||
| At1g20760 | 1019 | 2 | EPS15 repeat, pfkB | |||
| At1g21630 | 1181 | 4 | EPS15 repeat | |||
| At3g20290 | 485 | 2 | ATP_GTP_A, EPS15, NLS_BP | |||
| At4g05520 | 514 | 1 | EPS15 repeat, dynamin family | |||
| At1g47550 | 861 | 1 | NLS_BP | |||
| At1g47560 | 1564 | 1 | NLS_BP | |||
| At3g59270 | 335 | 1 | - | |||
| At5g41840 | 540 | 1 | F-box domain | |||
| Group II | ||||||
| At1g02270 | 484 | 1 | - | |||
| At5g54130 | 232 | 1 | - | |||
| At5g46830 | 511 | 1 | bHLH | |||
| At1g05150 | 808 | 1 | Zinc finger, TPR/put O-GlcNAc transferase | |||
| At2g32450 | 802 | 1 | Zinc finger, TPR/put O-GlcNAc transferase | |||
| At2g35380 | 336 | 1 | Plant peroxidase | sc (.960) | ||
| At1g20490 | 530 | 1 | AMP-dependent synthetase and ligase | |||
| At1g20510 | 546 | 1 | - | ch (.747) | ||
| At1g21540 | 550 | 1 | AMP-dependent synthetase and ligase | |||
| At1g23160 | 578 | 1 | - | |||
| At3g04860 | 289 | 1 | - | ch (.666) | ||
| At5g28150 | 289 | 1 | - | ch (.651) | ||
| At1g44780 | 471 | 1 | NLS_BP | |||
| At3g01780 | 1192 | 1 | - | |||
| At3g02270 | 676 | 1 | eIF4-gamma/eIF | |||
| At5g64060 | 356 | 1 | No apical meristem protein | |||
| At4g05110 | 394 | 1 | Nucleoside_tran | |||
| At4g05120 | 418 | 1 | Nucleoside_tran | |||
| At4g05140 | 419 | 1 | Nucleoside_tran | |||
| At5g22840 | 538 | 1 | Eukaryotic protein kinase | |||
| At5g12120 | 619 | 1 | Ubiquitin-associated domain | ch (.811) | ||
| At1g64850 | 162 | 1 | - | |||
| At3g46220 | 804 | 1 | Biotin operon repressor | |||
| At3g44330 | 565 | 1 | - | sc (.697) | ||
| At5g21130 | 281 | 1 | ATP synthase alpha and beta subunit | |||
| At3g14270 | 1791 | 1 | FYVE, PIP5K | |||
| At3g32010 | 603 | 1 | ATHILA | |||
| At3g32880 | 503 | 1 | ATHILA | |||
| At3g32970 | 289 | 1 | ATHILA | |||
| At4g08080 | 561 | 1 | - | |||
| At4g08050 | 1428 | 1 | - | |||
| At4g03790 | 1064 | 1 | ATHILA | |||
| At1g80680 | 1032 | 1 | Aminoacyl-transfer RNA syn class II | |||
| At2g30110 | 1080 | 1 | UBACT, ThiF_family | |||
| At5g46360 | 260 | 1 | Potassium channel/KCO1-like | |||
| At5g46370 | 443 | 1 | Potassium channel/KCO1-like | |||
| At5g55630 | 363 | 1¶ | Potassium channel | ch (.772) | KCO1 | [31] |
| At2g35800 | 844 | 1 | Mitoch_carrier | |||
| At5g07320 | 479 | 4 | Mitoch_carrier, Graves disease | |||
| At5g51050 | 487 | 2 | Mitoch_carrier, Adptrnslcase | ch (.621) | ||
| At5g61810 | 476 | 4 | Mitoch_carrier | |||
| At1g33790 | 745 | 1 | Jacalin-related lectin domain | |||
| At5g28340 | 390 | 2 | PPR repeats | |||
| At5g28380 | 283 | 2 | PPR repeats | |||
| At5g58670 | 559 | 1 | PIPLC | AtPLC1 | [29,42] | |
| At4g26700 | 687 | 1 | Calponin homology | ATFIM1 | [25] | |
| At5g55400 | 714 | 1 | Calponin homology | |||
| Group III | ||||||
| At1g03960 | 534 | 1 | /protein phosphatase 2A group | |||
| At5g44090 | 538 | 2 | /protein phosphatase 2A group | [58] | ||
| At1g54450 | 535 | 2 | /protein phosphatase 2A group | |||
| At5g28850 | 536 | 3 | /protein phosphatase 2A group | |||
| At5g28900 | 536 | 3 | /protein phosphatase 2A group | |||
| At5g18580 | 464 | 3 | NLS_BP | |||
| At5g49480 | 160 | 3 | /NaCl-inducible Ca2+ binding protein-like | AtCP1 | [35] | |
| At1g32410 | 1048 | 1 | PPR repeats | sc (.813) | ||
| At3g18430 | 175 | 1 | - | |||
| At1g64480 | 214 | 3 | Recoverin | CBL8 | [13] | |
| At5g24270 | 222 | 3 | - | CBL4 | [51] | |
| At4g33000 | 246 | 3 | Recoverin/CBL-like | sc (.931) | ||
| At4g16350 | 227¥ | 3# | - | CBL6 | [13] | |
| At5g55990 | 226 | 3 | - | CBL3 | [23] | |
| At4g26560 | 214 | 3 | Recoverin | CBL7 | [13] | |
| At4g26570 | 226 | 3 | - | CBL2 | [23] | |
| At4g17615 | 252 | 3 | Recoverin | CBL1 | [23] | |
| At5g47100 | 213 | 2 | Recoverin | CBL9 | [13] | |
| At4g01420 | 192 | 3 | - | CBL5 | [13] | |
| At1g54530 | 127 | 1 | - | |||
| At2g44310 | 142 | 2 | - | |||
| At5g28830 | 324 | 2 | - | |||
| At5g22760 | 1516 | 1 | PHD-finger, DDT domain | |||
| At4g38810 | 375 | 2 | - | |||
| At4g13440 | 154 | 2 | - | |||
| At4g32060 | 498 | 4 | - | |||
| At4g39420 | 781 | 1 | - | |||
| At1g29020 | 1062 | 4 | - | sc (.939) | ||
| At2g34020 | 462 | 2 | sc (.965) | |||
| At2g34030 | 423 | 2 | mt (.648) | |||
| At1g53210 | 574 | 2 | - | sc (.907) | ||
| At4g25970 | 628 | 2 | PS_Dcrbxylase, Porpobil_deam | ch (.849) | ||
| At5g57190 | 615 | 4 | PS_Dcrbxylase, Porpobil_deam | |||
| At2g46600 | 135f | 1 | - | KIC | Unpublished | |
| At4g27280 | 130 | 1 | - | |||
| At5g54490 | 127 | 1 | - | |||
| At3g17470 | 570 | 2 | - | |||
| At5g62250 | 549 | 1 | - | |||
| At4g27790 | 345 | 6 | ER_TARGET | sc (.941) | ||
| At5g08580 | 391 | 4 | - | sc (.631) | ||
| Group IV | ||||||
| At1g05990 | 150 | 4 | - | |||
| At4g03290 | 154 | 4 | - | |||
| At2g43290 | 169 | 4 | - | |||
| At3g59440 | 195 | 4 | - | sc (.844) | ||
| At3g59450 | 148 | 1 | - | |||
| At3g07490 | 153 | 4 | - | |||
| At4g12860 | 152 | 4 | - | |||
| At1g18210 | 170 | 4 | - | |||
| At1g73630 | 163 | 4 | - | |||
| At1g66400 | 157 | 4 | Calflagin | |||
| At5g37770 | 161 | 4 | - | TCH2 | [20] | |
| At2g36180 | 146 | 4 | - | |||
| At5g17470 | 146 | 4 | Calflagin | |||
| At3g03400 | 137 | 3 | - | |||
| At3g03410 | 131 | 4 | - | |||
| At2g15680 | 187 | 4 | Recoverin | ch (.725) | ||
| At1g21550 | 155 | 3 | - | |||
| At4g20780 | 191 | 3 | - | |||
| At5g44460 | 181 | 3 | - | |||
| At1g12310 | 148 | 3 | - | |||
| At1g62820 | 148 | 3 | - | |||
| At1g66410 | 149 | 4 | EPS15 repeat | CaM4 | [17] | |
| At5g37780 | 149 | 4 | - | CaM1 | [16] | |
| At2g27030 | 149 | 4 | EPS15 repeat | CaM5 | [17] | |
| At2g41110 | 149 | 4 | EPS15 repeat | CaM2 | [16] | |
| At3g56800 | 149 | 4 | EPS15 repeat | CaM3 | [15] | |
| At3g43810 | 149 | 4 | EPS15 repeat | CaM7 | [18] | |
| N/A | 149 | 4 | EPS15 repeat | CaM6 | [17] | |
| At2g41090 | 191 | 4 | - | CaBP-22 | [17] | |
| At3g22930 | 173 | 4 | - | mt (.702) | ||
| At4g14640 | 151 | 4 | - | CaM8 | [18] | |
| At2g41100 | 324 | 6 | - | TCH-3 | [20] | |
| At3g51920 | 151 | 4 | - | CaM9 | [18] | |
| At3g50360 | 169 | 4 | - | mt (.609) | Centrin | [21] |
| At4g37010 | 167 | 4 | /caltractin(centrin)-like | mt (.809) | ||
| At1g18530 | 157 | 4 | Calflagin | |||
| At3g25600 | 161 | 4 | Calflagin | |||
| At1g32250 | 166 | 4 | Calflagin, recoverin | |||
| At3g03000 | 165 | 4 | Calflagin | |||
| At2g41410 | 216 | 4 | - | ch (.770) | PM129 | [64] |
| At3g10190 | 209 | 4 | - | |||
| At3g03430 | 83 | 2 | Recoverin/pollen allergenBra r II | |||
| At5g17480 | 83 | 2 | /Calcium-binding protein in pollen | APC1 | [36] | |
| At1g76640 | 159 | 4 | - | |||
| At1g76650 | 177 | 3 | - | |||
| At3g01830 | 146 | 2 | - | |||
| At3g50770 | 205 | 4 | - | ch (.970) | ||
| At5g42380 | 185 | 3 | - | ch (.742) | ||
| At3g29000 | 194 | 2 | S-100/ICaBP type CBP | sc (.954) | ||
| At5g39670 | 193 | 2 | - | sc (.877) | ||
| At3g47480 | 183 | 2 | Recoverin | sc (.956) | ||
| At1g24620 | 186 | 4 | Calflagin | |||
| At3g24110 | 229 | 2 | - | |||
| At4g26470 | 248 | 4 | - | |||
| Group V | ||||||
| At2g27480 | 186 | 2 | - | |||
| At3g10300 | 330 | 2 | - | |||
| At5g04170 | 354 | 2 | - | |||
| Group VI | ||||||
| At1g09090 | 838 | 2 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohB | [26] | |
| At4g25090 | 863 | 2 | Cytb-245 hc, Ferric_reduct/rboh-like | |||
| At5g51060 | 905 | 1 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohC | [26] | |
| At5g07390 | 902 | 1 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohA | [26] | |
| At5g47910 | 921 | 2 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohD | [26] | |
| At1g64060 | 929 | 1 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohF | [26,27] | |
| At1g19230 | 926 | 1 | Cytb-245 hc, Ferric_reduct/rboh-like | RbohE | [26] | |
| At3g45810 | 835 | 1 | Cytb-245 hc, Ferric_reduct/rboh-like | |||
| At5g60010 | 839 | 2 | Cytb-245 hc, Ferric_reduct/rboh-like | |||
| At2g47860 | 635 | 1 | PTM, NPH3 | ch (.809) | ||
| At3g44820 | 661 | 1 | BTB/POZ domain | |||
| At5g05180 | 432 | 1 | - | |||
| At5g13260 | 576 | 1 | - | ch (.666) | ||
| At5g13960 | 624 | 1 | SET-domain of transcriptional regulators | |||
| At3g05310 | 648 | 2 | Small GTPase | |||
| At5g27540 | 648 | 2 | Small GTPase | |||
| At3g63150 | 676 | 1 | Small GTPase | |||
| At2g20800 | 582 | 1 | Pyr_redox | |||
| At4g05020 | 583 | 1 | FAD_pyr_redox | |||
| At4g28220 | 559 | 1 | Pyr_redox | mt (.880) | ||
| At4g08000 | 609 | 1 | En/Spm-like transposon proteins | |||
| At4g03560 | 724 | 2 | Cation (ca, na) pore region, HLH_Myc | |||
| At1g02960 | 553 | 1 | PTS_HPR_SER, NLS | |||
| At5g59230 | 186 | 1 | - | |||
| At4g39560 | 343 | 1 | F-box, Kelch repeat | |||
| At4g26080 | 434 | 1 | Protein phosphatase 2C subfamily | ABI1 | [34,78] | |
| At5g07440 | 411 | 1 | NAD-binding site, GLFV_dehydrog | mt (.680) | GDH2 | [32] |
| At1g76250 | 434 | 1 | - | sc (.648) | ||
| At5g44620 | 519 | 1 | Cytochrome P450 enzyme, HLH_Myc | |||
| At1g01280 | 510 | 1 | Cytochrome P450 enzyme | sc (.725) |
The table is arranged by groups.*Domains as identified by InterProScan [40]. †Remarks are from MAtDB [39] or the reference. ‡Based on TargetP predictions [44]. Only signals with scores of 0.600 or above were included. mt, mitochondria; ch, chloroplast; sc, secretory pathway. §EF-hand motifs identified by only one database in InterProScan are in bold. ¶Only one EF-hand found by InterProScan but two identified by Czempinski et al. [31]. ¥Kudla et al. [23] report different protein sequence whose size and number of EF-hand domains varies from the MIPS database information. #There is a discrepancy between the MIPS database sequence and the experimentally determined size. N/A, not available; Adptrnslcase, adenine nucleotide translocator 1; ATHILA, Arabidopsis retrotransposon (ATHILA) ORF-1; ATP_GTP_A, ATP/GTP-binding site motif A; bHLH, basic helix-loop-helix dimerization domain; Calflagin, flagellar calcium-binding protein; Cytb-245 hc, cytochrome B-245 heavy chain; EFG_C, elongation factor G, carboxyl terminus; ER_TARGET, endoplasmic reticulum targeting seq; FAD_pyr_redox, FAD-dependent pyridine nucleotide-disulfide oxidoreductase; Ferric_reduct, ferric reductase-like transmembrane component; FYVE, FYVE An-finger, rabphilin/VPS27/FABI type; GatC, glutamyl-tRNA amidotransferase C subunit; GLFV_dehydrog, glutamate/leucine/phenylalanine/valine dehydrogenase; GTP_EFTU_D2, elongation factor Tu domain 2; HLH_Myc, Myc-type helix-loop-helix dimerization domain; Mitoch_carrier, mitochondrial energy transfer proteins; NLS_BP, nuclear localization signal, bipartite; Nucleoside_tran, delayed-early response protein/equilibrative nucleoside transporter; PIP5K, phosphatidylinositol-4-phosphate-5-kinase; PIPLC, phosphatidylinositol-specific phospholipase; pfkB, pfkB family of carbohydrate kinases; Porpobil_deam, porphobilinogen deaminase; PS_Dcrbxylase, phosphatidylserine decarboxylase; PTM, phosphopantetheine attachment site; PTS_HPR_SER, serine phosphorylation site in HPr protein; Pyr_redox, pyridine nucleotide-disulfide oxidoreductase; TM, transmembrane; ThiF_family, UBA/THIF-type NAD/FAD-binding fold; TPR, tetratricopeptide repeat; UBACT, ubiquitin-activating enzyme; UIM, ubiquitin-interacting motif.