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. 2002 Feb;22(4):1266–1275. doi: 10.1128/MCB.22.4.1266-1275.2002

FIG. 4.

FIG. 4.

Nuclear accumulation of rpL12 is competed specifically by an importin 11 transport substrate. BHK cells were microinjected into the cytoplasm with mixtures containing a GFP-tagged import cargo (3.75 μM), TRITC-labeled dextran (1 mg/ml), and, where indicated, a competitor protein (50 μM). Cells were incubated for 10 min at 37°C before fixation. Thirty to 40 cells/sample were injected with similar results. (A) Panels a, d, and g show TRITC fluorescence (Inj marker); panels b, e, and h show GFP fluorescence from L12-GGH6; and panels c, f, and i show DNA staining (DAPI). Competitor proteins used were myc-UbcM2-H6 (UbcM2) and zz-BIB-H6 (BIB). Bar, 10 μm. (B) Panels j and m show TRITC fluorescence (Inj marker); panels k and n show GFP fluorescence from L23-GFP; and panels l and o show DNA staining (DAPI). The competitor protein used was myc-UbcM2-H6 (UbcM2). (C) Panels p, s, and v show TRITC fluorescence (Inj marker); panels q, t, and w show GFP fluorescence from GGNLS; and panels r, u, and x show DNA staining (DAPI). The competitor proteins used were myc-UbcM2-H6 (UbcM2) and zz-BIB-H6 (BIB).