TABLE 2.
Semidominance of SPO11 alleles
| Test allele | Results for configurationa
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A (Test allele with SPO11)
|
B (Test allele with SPO11 [both tagged])
|
C (Test allele with SPO11 [test allele tagged])
|
D (Test allele with SPO11 [SPO11 tagged])
|
||||||||||
| Frequency of His+b | Viability (%) (n)c | Strain | Frequency of His+ | Viability (%) (n) | Strain | Frequency of His+ | Viability (%) (n) | Strain | Frequency of His+ | Viability (%) (n) | Strain | ||
| Wild type | 18.9 ± 3.6 | 98.0 (100) | SKY534 | 11.8 ± 5.8 | 97.1 (140) | SKY334 | |||||||
| spo11Δ | 10.4 ± 6.8 | 96.3 (100) | SKY486 | 8.4 ± 2.5 | 94.5 (160) | SKY344 | |||||||
| Y135F | 9.9 ± 3.9 | 94.7 (99) | SKY546 | 4.4 ± 2.2 | 51.1 (162) | SKY335 | 14.2 ± 4.0 | 95.8 (100) | SKY533 | 0.12 ± 0.046 | 2.7 (100) | SKY545 | |
| E233A | 9.6 ± 5.0 | 95.0 (100) | SKY487 | 11.1 ± 5.4 | 86.0 (100) | SKY587 | 13.8 ± 6.6 | 98.0 (100) | SKY588 | 0.16 ± 0.045 | 0.50 (100) | SKY458 | |
| D288N | 8.7 ± 0.75 | 97.3 (100) | SKY488 | 1.1 ± 1.0 | 44.0 (138) | SKY337 | 7.5 ± 2.3 | 99.0 (100) | SKY529 | 0.18 ± 0.11 | 2.5 (100) | SKY459 | |
| D290A | 13.1 ± 2.2 | 98.5 (100) | SKY538 | 7.1 ± 1.9 | 70.0 (109) | SKY336 | 7.8 ± 2.5 | 97.0 (100) | SKY530 | 7.03 ± 1.3 | 94.8 (100) | SKY539 | |
a
Each of the indicated test alleles (with or without the HA3His6 tag) was crossed to wild-type SPO11 (with or without the tag), as indicated (configuration).
b
Meiotic intragenic recombination at his4::LEU2 was measured as the frequency of His+ prototrophs per viable cell after 8 h in SPM at 30°C (frequency × 103; mean ± standard deviation of at least three determinations).
c
Spore viability was assayed by dissection of the indicated number (n) of four-spored asci produced after ≥24 h in SPM at 30°C.