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RANKL-induced association of endogenous TAK1 with TRAF6. 293-RANK cells were treated with 1,000 ng of RANKL per ml for indicated times. Cell extracts were immunoprecipitated with anti-TAK1 antibody. Coprecipitated TRAF6 was detected by immunoblotting with anti-TRAF6 antibody (top panel). The amounts of TAK1 in each immune complex were determined by immunoblotting (middle panel). Total amounts of TRAF6 were determined by immunoblotting in cell lysates (bottom panel).
