Table 6.

Inhibitory activity of selected derivatives in the infectious EBOV and MARV assay

#	Hela CC50 (μM)	EBOV EC50a	SI EBOVb	MARV EC50a	SI MARVb
31	8.1±0.2	0.05±0.01	162	ndc	ndc
33	7.2±0.2	0.27±0.04	27	ndc	ndc
35	8.2±0.3	0.07±0.01	117	1.3 ± 1.6	6
36	8.3±0.4	0.03±0.00	330	ndc	ndc
41	4.6±0.1	0.04±0.01	120	0.38 ± 0.44	12
3	3.9±0.1	0.08±0.02	48	1.2 ± 0.2	3
Remdesivir	> 12	0.12±0.04	> 104	0.18 ± 0.07	70

^aDose–response curves were used to calculate EC₅₀ values using the four-parameter variable slope nonlinear regression analysis in GraphPad Prism 10.2.3. Data are presented as the mean EC₅₀ from three replicates ± standard deviation.

^bSelectivity indices (SI) are calculated as a ratio of CC₅₀/EC₅₀ where CC₅₀ is cytotoxicity measured in a Cell Titer-Glo Luminescent Cell Viability Assay.

^cnd: not determined.