4.

Enzyme-mediated phase reorganization in multiphase coacervate droplets. a) Schematic illustrating the morphological differences between the pAsp multiphase system at ∼pH 4.5 and ∼pH 6, showing the approximate locations of polyelectrolytes and enzymes at each pH extreme. b) Confocal microscopy images of the pAsp multiphase system over time as pH increases due to urease-mediated hydrolysis of urea (5 mM), producing ammonia and driving a pH increase from ∼pH 4.5 to ∼pH 6. Dashed lines indicate the positions of the corresponding line profiles shown in c. c) Line profiles showing the redistribution of fluorescently labeled components and the change in phase morphology over the course of the reaction (profiles correspond to dashed lines in b. The pH of the coacervate mixture was measured using a pH probe before and after the reaction to determine the extent of the pH change. Scale bars: 10 μm.