FIG. 9.

Antisense blocking of RhoH increases Rac1-induced activity. 293 or Jurkat cells were cotransfected with reporter genes for IFN-γ-Luc (2 μg) and Rac1L61 (2 μg), with or without an αs-RhoH vector (2 or 4 μg). At 24 h after transfection, cell were processed either for luciferase activity measurement or for Northern analysis to assess endogenous levels of RhoH mRNA. (a) In 293 cells, Rac1L61 induced IFN-γ activation and no difference between activity levels was caused by αs-RhoH expression. (b) In Jurkat cells, RacL61 induces an equivalent level of IFN-γ activation. In cells transfected with αs-RhoH, there was an increase in IFN-γ activity proportionate to the reduction in the endogenous RhoH mRNA level. The experiment was repeated two times, and a typical result is shown.