FIG. 4.

Recombinant human CGI-75 protein binds DNA in a non-sequence-specific manner. An autoradiogram from a gel mobility shift assay (see Materials and Methods) using recombinant GST-purified CGI-75 protein and an mtDNA probe containing the mitochondrial LSP is shown. Plus and minus signs indicate the presence and absence of CGI-75 protein and the nonspecific competitive inhibitor poly(dI-dC) in the sample, respectively. Location of the unbound DNA probe is indicated by the arrow, and the position of the retarded CGI-75-bound probe is indicated by the arrowhead. Thirty nanograms of poly(dI-dC) was added where indicated, and the amount of CGI-75 in each reaction is indicated at the top of the figure (numbers indicate nanograms of CGI-75 added).