FIG. 6.

Dynamic redistribution of D1 during gametogenesis. Adult gonads were stained for DNA and D1 protein and processed for confocal microscopy. (A) Different views of female ovaries: global and more detailed views of the anterior part of an ovariole (a and b, respectively) showing the germarium, ovarian chambers (c and d), and DNA, D1, and merged images of a late-stage oocyte (e, f, and g, respectively). The germarium (ge), follicle cells (fc), nurse cells (nc), the karyosome (k), and oocyte (oo) are indicated. A similar analysis is shown for male testes (B). D1 was revealed by immunofluorescence as described above or by peroxidase staining (d). A diagram adapted from one by Lindsley and Tokuyasu (37) is shown (a) and represents the first half-gyre of a testis with the different spermatocyte cysts that form single-cell layers near the testicular walls. A whole gonad is shown (b) together with a close-up view of the D1 signal in cells from the central part of the testis (top inset). In the germarium (d and e and diagrammed in c), D1 localizes to bright foci in both apical cells (ac) and spermatogonia (sg). Cells situated just below correspond to spermatocytes (sp) in which the protein has redistributed to fill most of the nucleoplasm (e, lower area; f, magnification of panel e).