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Use of a direct-repeat substrate to measure mitotic recombination. The substrate contains leu2 heteroalleles separated by a copy of the URA3 gene. Leu+ Ura+ recombinants can be generated by intrachromatid or sister chromatid conversion or by unequal sister chromatid exchange. Ura− recombination events can occur by an intrachromatid or unequal sister chromatid crossover, by SSA, or by replication slip mispairing.
