FIG. 7.

Physical assays for meiotic recombination. (A) The hot spot created by insertion of the LEU2 gene at HIS4 in the SK1 strain background is shown. The end of the LEU2 gene contains a site for meiosis-specific DSBs flanked by restriction sites that can be used to distinguish between DSB fragments, and the two parental and two recombinant fragments. The white and black boxes indicate insertions of heterologous sequences used to distinguish the parental molecules by using strand-specific probes. (B) Three types of joint molecules are expected: Mom-Mom intersister joint molecules, Dad-Dad intersister joint molecules, and Mom-Dad interhomologue joint molecules. Size and hybridization to strand specific probes distinguish the three types of joint molecules. (C) Cartoon of a neutral-neutral two-dimensional gel showing separation of joint molecules from the parental and recombinant fragments. The interhomologue joint molecules are more abundant than the intersister joint molecules in wild-type cells. (Adapted from Fig. 1 of reference 329 with permission)